Bioinformatically designed complex oligonucleotide probe sets for FISH

Welcome to the Oligopaints website! Here, you will find database files listing the genomic locations of Oligopaints probes in the C. elegans (ce6), Drosophila (dm3), Arabidopsis (tair10), mouse (mm9), and human (hg19) genomes. We also provide a suite of python scripts for working with these files and a guide that explains how the scripts work in detail. Finally, there are protocols to guide you through the synthesis of an Oligopaints probe set once you have designed and ordered your oligo library. Check back periodically for database and protocol updates. Happy FISHing!!


Oligopaints and Related Technologies Publications

Beliveau BJ, Apostolopoulos N, Wu C-t. Visualizing genomes with Oligopaint FISH probes. Curr Protocols Mol Biol 2014 14.23. PMID: 24510436 PMCID: PMC3928790.

Schmidt T, Beliveau B, Uca Y, Theilmann M, Da Cruz F, Wu C-t, Shih W. Scalable amplification of strand subsets from chip-synthesized oligonucleotide libraries. Nat Commun 2015 6:8634. doi: 10.1038/ncomms9634. PMID: 26567534. PMCID: PMC4660042.

Murgha Y, Beliveau B, Semrau K, Schwartz D, Wu C-t, Gulari E, Rouillard J-M. Combined in vitro transcription and reverse transcription method to amplify and label complex synthetic oligonucleotide probe libraries. Biotechniques 2015 58:301-7. PMID: 26054766.


Last updated 07/14/2016 by CT Wu


Contact: twu@genetics.med.harvard.edu







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