PCR Primers and Conditions

PCR CONDITIONS (LAMIN A/C)
Primers	1st Denature	Denature	Annealing	Extention	Final Extention	Cycles
1f & 1r	94,4 min	94,30	67,30	72,30	72,4min	35
2f & 2r	94,4min	94,30	58,30	72,45	72,4min	35
3f & 4r	94,4min	94,30	60,30	72,45	72,4min	30
4f & 5r	94,4min	94,30	59,30	72,45	72,4min	35
6f &7r	94,4min	94,30	58,30	72,45	72,4min	35
7f & 8r	94,4min	94,30	64,30	72,45	72,4min	30
9f & 11r	94,4min	94,30	67,30	72,45	72,4min	30
12F04 & 12R04	94,4min	94,30	58,30	72,45	72,4min	35