TPM1 exons, primers, and amplimers

Exons, Primers, and Amplimers of alpha-Tropomyosin

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TPM1 Exon 1

CCGCGCGCTCGCCCCGCCGCTCCTGCTGCAGCCCCAGGGCCCCTCGCCGC
CGCCACCATGGACGCCATCAAGAAGAAGATGCAGATGCTGAAGCTCGACA
AGGAGAACGCCTTGGATCGAGCTGAGCAGGCGGAGGCCGACAAGAAGGCG
GCGGAAGACAGGAGCAAGCAG

length: 171 bp

EXON	from	to
genomic DNA	1001	1171
mRNA

Primers for Exon 1
1F attggctgtcttgaggaatgcggt (24 bp)

1R taagcgtcctcagaccagactaag (24 bp)

**Primers for Exon 1**
1F	attggctgtcttgaggaatgcggt	(24 bp)
1R	taagcgtcctcagaccagactaag	(24 bp)

(Annealing temp.: 58° C.)

Amplimer for Exon 1

ATTGGCTGTCTTGAGGAATGCGGTCGCCCCCTTGGGAAAGTACATATCTG
GGAGAAGCAGGCGGCTCCGCGCTCGCACTCCCGCTCCTCCGCCCGACCGC
GCGCTCGCCCCGCCGCTCCTGCTGCAGCCCCAGGGCCCCTCGCCGCCGCC
ACCATGGACGCCATCAAGAAGAAGATGCAGATGCTGAAGCTCGACAAGGA
GAACGCCTTGGATCGAGCTGAGCAGGCGGAGGCCGACAAGAAGGCGGCGG
AAGACAGGAGCAAGCAGGTCTGCGCCTCCCCGGCCCTGCGCCCGCGCCCA
GAGCGCCGGGACTCGAGCCTGGCACCCCCGGCTGGATCCCCACCCCGAGG
ACTCGGGGAGCCACCACCCTACCCCCACCACCACCCAGGGCGGCCAGGGC
GCGCGTCTGGAAAGAAGGAAGGGAAACGCAAGAGCCAGGCTTAGTCTAAC
TTAGTCTGGTCTGAGGACGCTTA

length: 473 bp

AMPLIMER	from	to
genomic DNA	905	1377

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TPM1 Exon 2

CTGGAAGATGAGCTGGTGTCACTGCAAAAGAAACTCAAGGGCACCGAAGA
TGAACTGGACAAATACTCTGAGGCTCTCAAAGATGCCCAGGAGAAGCTGG
AGCTGGCAGAGAAAAAGGCCACCGAT

length: 126 bp

EXON	from	to
genomic DNA	2255	2380
mRNA

Primers for Exon 2
2F ctgtctttccctctgtctct (20 bp)

2R tgcaggtgtccagcaggaaa (20 bp)

**Primers for Exon 2**
2F	ctgtctttccctctgtctct	(20 bp)
2R	tgcaggtgtccagcaggaaa	(20 bp)

(Annealing temp.: 56° C.)

Amplimer for Exon 2

CTGTCTTTCCCTCTGTCTCTCCCGCTGTCCCTGTCCTTCTGGTTCTGTGC
ACCCACACCCCTCCCCTTCGGGATCACGCTGCCTGCTGCACCCCCCTCCC
TCCCTGTACCCCCTGGCCAACTCCCAGCTGGAAGATGAGCTGGTGTCACT
GCAAAAGAAACTCAAGGGCACCGAAGATGAACTGGACAAATACTCTGAGG
CTCTCAAAGATGCCCAGGAGAAGCTGGAGCTGGCAGAGAAAAAGGCCACC
GATGTAAGTGCACGCTCACACTGCTTCCCTCACCTCTTGCCTGCGTGGCC
ACTCCGGGGTCACCACAGGGCTGGAGAGCAATGAAGGAAGTTTACCTTTT
CCTGCTGGACACCTGCA

length: 367 bp

AMPLIMER	from	to
genomic DNA	2128	2494

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TPM1 Exon 3

GCTGAAGCCGACGTAGCTTCTCTGAACAGACGCATCCAGCTGGTTGAGGA
AGAGTTGGATCGTGCCCAGGAGCGTCTGGCAACAGCTTTGCAGAAGCTGG
AGGAAGCTGAGAAGGCAGCAGATGAGAGTGAGAG

length: 134 bp

EXON	from	to
genomic DNA	15213	15346
mRNA

Primers for Exon 3
3F tgagggcttgtaatgcacttaaag (24 bp)

3R ccaggggactgaaaaattcttaaa (24 bp)

**Primers for Exon 3**
3F	tgagggcttgtaatgcacttaaag	(24 bp)
3R	ccaggggactgaaaaattcttaaa	(24 bp)

(Annealing temp.: 58° C.)

Amplimer for Exon 3

TGAGGGCTTGTAATGCACTTAAAGGTAGCTCACCACAAGACAGTCTTCCT
ATCTGAAATCAGCATTGCAGTCCCAGCCATTTCCTGAAGCTACCACCCTC
ACTTTCTCCCCAACTCTGAAATGCTTTTCACTCTCTACCTAGGCTGAAGC
CGACGTAGCTTCTCTGAACAGACGCATCCAGCTGGTTGAGGAAGAGTTGG
ATCGTGCCCAGGAGCGTCTGGCAACAGCTTTGCAGAAGCTGGAGGAAGCT
GAGAAGGCAGCAGATGAGAGTGAGAGGTGAGAATGCCTCATCAGCCATCT
TTTGCAGCTGCCTTTCCTGGTGGAATAAACCGGAGGGCTCCTGTGATCTT
TGAGGTTTAAGAATTTTTCAGTCCCCTGG

length: 379 bp

AMPLIMER	from	to
genomic DNA	15071	15449

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TPM1 Exon 4

AGGCATGAAAGTCATTGAGAGTCGAGCCCAAAAAGATGAAGAAAAAATGG
AAATTCAGGAGATCCAACTGAAAGAGGCCAAGCACATTGCTGAAGATGCC
GACCGCAAATATGAAGAG

length: 118 bp

EXON	from	to
genomic DNA	17791	17908
mRNA

Primers for Exon 4
4F cacaagccacagcagtgcagtg (22 bp)

4R atggcctcactcgggaatcaag (22 bp)

**Primers for Exon 4**
4F	cacaagccacagcagtgcagtg	(22 bp)
4R	atggcctcactcgggaatcaag	(22 bp)

(Annealing temp.: 60° C.)

Amplimer for Exon 4

CACAAGCCACAGCAGTGCAGTGTGCATTTGGGAAGTTCAGCTCTAAATCT
TGGGTTTTCTTGCTTGTCTTTCTTTTCAGAGGCATGAAAGTCATTGAGAG
TCGAGCCCAAAAAGATGAAGAAAAAATGGAAATTCAGGAGATCCAACTGA
AAGAGGCCAAGCACATTGCTGAAGATGCCGACCGCAAATATGAAGAGGTC
AGATCCTGGGGCCCAAAGCCTTGTGGACACCCAGCAGTGGCCTTCAGGAA
GCCAGGGAGCAATGTACAGTCTTGATTCCCGAGTGAGGCCAT

length: 292 bp

AMPLIMER	from	to
genomic DNA	17712	18003

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TPM1 Exon 5

GTGGCCCGTAAGCTGGTCATCATTGAGAGCGACCTGGAACGTGCAGAGGA
GCGGGCTGAGCTCTCAGAAGG

length: 71 bp

EXON	from	to
genomic DNA	19097	19167
mRNA

Primers for Exon 5
5F acccccatgcccttctgttacaca (24 bp)

5R caaaaggagcccggagctgaaaca (24 bp)

**Primers for Exon 5**
5F	acccccatgcccttctgttacaca	(24 bp)
5R	caaaaggagcccggagctgaaaca	(24 bp)

(Annealing temp.: 56° C.)

Amplimer for Exon 5

ACCCCCATGCCCTTCTGTTACACAAAGCTTGCAAGACCCATGGTGTGTGT
GTTGTGTCTTCCTGCTGCAGGTGGCCCGTAAGCTGGTCATCATTGAGAGC
GACCTGGAACGTGCAGAGGAGCGGGCTGAGCTCTCAGAAGGGTAAGCGGG
CCCGGCGCCAGGAGGCCACGAATGGGGTGCTGCAGAGCAGTGACTAAACA
GCATGACCTTCTGGCAGCTGCACATTACCTGTTTCAGCTCCGGGCTCCTT
TTG

length: 253 bp

AMPLIMER	from	to
genomic DNA	19027	19279

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TPM1 Exon 6

CAAATGTGCCGAGCTTGAAGAAGAATTGAAAACTGTGACGAACAACTTGA
AGTCACTGGAGGCTCAGGCTGAGAAG

length: 76 bp

EXON	from	to
genomic DNA	19941	20016
mRNA

Primers for Exon 6
6F gcagtgttcctggaaaacct (20 bp)

6R ctctgctttcttgcctattg (20 bp)

**Primers for Exon 6**
6F	gcagtgttcctggaaaacct	(20 bp)
6R	ctctgctttcttgcctattg	(20 bp)

(Annealing temp.: 56° C.)

Amplimer for Exon 6

GCAGTGTTCCTGGAAAACCTAAACATTTTAATACCTGATGATTTGGCTAC
TTTAAGGCAGCCCTTCGTCTCTAGGACTCAGTTTTCATTTTTTCCCATCC
CTCTCCTTTTTCTCTCCTCCTTCCTTTGGCTTGTCTCCCACCCTTTCTGC
CTCTGATCGAAAACATTAGCAAATGTGCCGAGCTTGAAGAAGAATTGAAA
ACTGTGACGAACAACTTGAAGTCACTGGAGGCTCAGGCTGAGAAGGTAGG
CCAGGAGGATGGTGTGGGGGAAAGGCATCTTTTAAGAGCTGCTCAAAAGA
GGCCCTGCCAGAAAGCAACACTTACAGTAGACATTTTAGTAAAATGGCAA
TATTGTGAGGTGATTTTTGGAGAGTTACTAGATAACAAATTCTTTTTTTT
AACTAGAGAATTTATTTTATGTTAGGGATATGAGGCATCAATTTAATTTA
AACCAAGTGCCAAGTGGATAAGTTATCTTGTTGTTATCCCATGTAAAACA
ATAGGCAAGAAAGCAGAG

length: 518 bp

AMPLIMER	from	to
genomic DNA	19772	20289

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TPM1 Exon 7

TACTCGCAGAAGGAAGACAGATATGAGGAAGAGATCAAGGTCCTTTCCGA
CAAGCTGAAGGAG

length: 63 bp

EXON	from	to
genomic DNA	20443	20505
mRNA

Primers for Exon 7
7F gtagattgagctgcagcctgacatc (25 bp)

7R gcgacctttgaatattcctgacttg (25 bp)

**Primers for Exon 7**
7F	gtagattgagctgcagcctgacatc	(25 bp)
7R	gcgacctttgaatattcctgacttg	(25 bp)

(Annealing temp.: 58° C.)

Amplimer for Exon 7

GTAGATTGAGCTGCAGCCTGACATCTGGAATGCTCTTTCTAATTACAGTA
CTCGCAGAAGGAAGACAGATATGAGGAAGAGATCAAGGTCCTTTCCGACA
AGCTGAAGGAGGTAATATGAGAGTTGTGGATGAAGCCAACTGGATTTTAA
ATGAGTTTGTTTTCATGGAACCGGTCAGGGCCTTTTCATTTTAAAGTTCC
AATGATCCAAGTCAGGAATATTCAAAGGTCGC

length: 232 bp

AMPLIMER	from	to
genomic DNA	20395	20626

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TPM1 Exon 8

GCTGAGACTCGGGCTGAGTTTGCGGAGAGGTCAGTAACTAAATTGGAGAA
AAGCATTGATGACTTAGAAG

length: 70 bp

EXON	from	to
genomic DNA	20804	20873
mRNA

Primers for Exon 8
8F tgccatttctcacagaggtgactg (24 bp)

8R gatgcccttgaattggaaaattgt (24 bp)

**Primers for Exon 8**
8F	tgccatttctcacagaggtgactg	(24 bp)
8R	gatgcccttgaattggaaaattgt	(24 bp)

(Annealing temp.: 58° C.)

Amplimer for Exon 8

TGCCATTTCTCACAGAGGTGACTGAAACTGACAAGTAGTTTCTGATCCAT
TTTATAGGTGATGTGCTTCATTTTCATCCTCTAGTTTTCCCTATGTTTGT
AGCTACAGGAAACATAAAACTTCCCAACTTTAACTCAAATAAATCATTAC
AGGCTGAGACTCGGGCTGAGTTTGCGGAGAGGTCAGTAACTAAATTGGAG
AAAAGCATTGATGACTTAGAAGGTAAGATCTTAAGTAGTGTTTTTAGTTT
AATCCTTATGGTTGAATACCAACCTGGCAAAACAATTTTCCAATTCAAGG
GCATC

length: 305 bp

AMPLIMER	from	to
genomic DNA	20652	20956

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TPM1 Exon 9

ACGAGCTGTACGCTCAGAAACTGAAGTACAAAGCCATCAGCGAGGAGCTG
GACCACGCTCTCAACGATATGACTTCCAT

length: 79 bp

EXON	from	to
genomic DNA	22292	22370
mRNA

Primers for Exon 9
9F caaccccttcatgcattgcc (20 bp)

9R tgctctagttcagcgtttac (20 bp)

**Primers for Exon 9**
9F	caaccccttcatgcattgcc	(20 bp)
9R	tgctctagttcagcgtttac	(20 bp)

(Annealing temp.: 56° C.)

Amplimer for Exon 9

CAACCCCTTCATGCATTGCCCTTTTCTTGCTGCTGTGTTGGGATGGTGCG
CGCACCACCCTCACTCACCCTCCATTTCTTGATCACTCTCCATGTTCTTG
CACCTCTGCCTTCCACTTCCTGGTCATAGACGAGCTGTACGCTCAGAAAC
TGAAGTACAAAGCCATCAGCGAGGAGCTGGACCACGCTCTCAACGATATG
ACTTCCATGTAAACGTTCATCCACTCTGCCTGCTTACACCCTGCCCTCAT
GCTAATGTAATAAACTCACCACCATGCCTTCCTTGCTCCCTAATCTCCAT
CTTTGCACTCTTGTGTTCACCCTTTTTGTCATAACCTAGAATAGTCATTG
TTTTTTCACTTTCTGCTAATATAAAGGCCAATTAGATTAAGTCTGTCTAT
ACAAACCATTTTCTTTTCAGAATCCGTTTATTTTGTAAACGCTGAACTAG
AGCA

length: 454 bp

AMPLIMER	from	to
genomic DNA	22163	22616

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TPM1 Exon 10

ATAAGTTTCTTTGCTTCACTTCTCCCAAGACTCCCTCGTCGAGCTGGATG
TCCCACCTCTCTGAGCTCTGCATTTGTCTATTCTCCAGCTGACCCTGGTT
CTCTCTCTTAGCATCCTGCCTTAGAGCCAGGCACACACTGTGCTTTCTAT
TGTACAGAAGCTCTTCGTTTCAGTGTCAAATAAACACTGTGTAAGCT

length: 197 bp

EXON	from	to
genomic DNA	24124	24320
mRNA

Primers for Exon 10
10F caagtgctctcatctattgg (20 bp)

10R agcagccaaacactaaccct (20 bp)

**Primers for Exon 10**
10F	caagtgctctcatctattgg	(20 bp)
10R	agcagccaaacactaaccct	(20 bp)

(Annealing temp.: 56° C.)

Amplimer for Exon 10

CAAGTGCTCTCATCTATTGGTTTGGTTTCCTTTCTTTTTTTTTTTTTTCT
CATTGTGCCACTTTTTTTTTCCTCCCACCTTTTTATCTTCACGCAGATAA
GTTTCTTTGCTTCACTTCTCCCAAGACTCCCTCGTCGAGCTGGATGTCCC
ACCTCTCTGAGCTCTGCATTTGTCTATTCTCCAGCTGACCCTGGTTCTCT
CTCTTAGCATCCTGCCTTAGAGCCAGGCACACACTGTGCTTTCTATTGTA
CAGAAGCTCTTCGTTTCAGTGTCAAATAAACACTGTGTAAGCTATTTCTG
TTTGCTATTCTTTTTACTTCTTATTTATTGACATTTTAGTTTCAACATTG
AATAAAACTACAAAGCTGCTTCACACATATGAGGGTTAGTGTTTGGCTGC
T

length: 401 bp

AMPLIMER	from	to
genomic DNA	24028	24428

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Last modified: April 24, 2006