TNNT2 exons, primers, and amplimers

Exons, Primers, and Amplimers of cardiac Troponin T

Jump to exon 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | top

TNNT2 Exon 2

acctgcactgacttttttctccttttggagggagagcagagaccatgtct
gacatagaagaggtggtggaagagtacgaggagga

length: 85 bp

EXON	from	to
genomic DNA	771	855
mRNA	7	91

Primers for Exon 2
TrpnT 2F acagctcatgaggggtggaacta (23 bp)

TrpnT 2R gtgctctgcctgggatctacaacc (24 bp)

**Primers for Exon 2**
TrpnT 2F	acagctcatgaggggtggaacta	(23 bp)
TrpnT 2R	gtgctctgcctgggatctacaacc	(24 bp)

(Annealing temp.: 65° C.)

Amplimer for Exon 2

acagctcatgaggggtggaactagattcaccctaggcctcgctgatctct
gtacaacgggggccagagctcttctgaggaaggcaggcttccctttgtac
ctgcactgacttttttctccttttggagggagagcagagaccatgtctga
catagaagaggtggtggaagagtacgaggaggagtgagtatctggagcat
cttgcctgagtggggtcctctcccgccgctgccctgacacctggtccagg
agcctcccagctgtccctcggattctgggtagaagtagctgtgtgtgttt
tgggcaccccgaggagagatatagccatttcctttccactcctgtacccc
tgggttgtagatcccaggcagagcac

length: 376 bp

AMPLIMER	from	to
genomic DNA	673	1048

Jump to exon 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | top

TNNT2 Exon 3

ggagcaggaag

length: 11 bp

EXON	from	to
genomic DNA	1914	1924
mRNA	92	102

Primers for Exon 3
TrpnT 3F atgagaacggcaggccaggctagtg (25 bp)

TrpnT 4R gtttgcctcaagacccgagcaacc (24 bp)

**Primers for Exon 3**
TrpnT 3F	atgagaacggcaggccaggctagtg	(25 bp)
TrpnT 4R	gtttgcctcaagacccgagcaacc	(24 bp)

(Annealing temp.: 65° C.)

Amplimer for Exon 3

atgagaacggcaggccaggctagtgggtgtcattgcaaggtgggcagggc
agcgtggactccactaggcaacaagggaaaagaaagggggattatctttg
gggaaaggccagtgtgtgcatgtgtgtgcaggcgtgtgtgtttgcatgtg
cttgtgtgcgagctactgacagtgtttcctgttgctctcagggagcagga
aggtaagcgtaaacgtgtgtactcatttggatcaaagacagcctggttcg
aaactgacccacctcttctctcttctcttgctgcctggacttctcttctg
agcagaagcagctgttgaaggtatgtgcctcaggcaggtggagagccaga
aaggggcaggctcagcaatgtggaaatcctgggagccctgggaaagcagc
tcatctgtccctgctcctcactctcagctgagcaagatcctccaacaaca
gtgccttctaaaatgggaatcagtcctacctgggttgctcgggtcttgag
gcaaac

length: 506 bp

AMPLIMER	from	to
genomic DNA	1723	2228

Jump to exon 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | top

TNNT2 Exon 4

aagcagctgttgaag

length: 15 bp

EXON	from	to
genomic DNA	2028	2042
mRNA	103	117

Primers for Exon 4
TrpnT 3F atgagaacggcaggccaggctagtg (25 bp)

TrpnT 4R gtttgcctcaagacccgagcaacc (24 bp)

**Primers for Exon 4**
TrpnT 3F	atgagaacggcaggccaggctagtg	(25 bp)
TrpnT 4R	gtttgcctcaagacccgagcaacc	(24 bp)

(Annealing temp.: 65° C.)

Amplimer for Exon 4

atgagaacggcaggccaggctagtgggtgtcattgcaaggtgggcagggc
agcgtggactccactaggcaacaagggaaaagaaagggggattatctttg
gggaaaggccagtgtgtgcatgtgtgtgcaggcgtgtgtgtttgcatgtg
cttgtgtgcgagctactgacagtgtttcctgttgctctcagggagcagga
aggtaagcgtaaacgtgtgtactcatttggatcaaagacagcctggttcg
aaactgacccacctcttctctcttctcttgctgcctggacttctcttctg
agcagaagcagctgttgaaggtatgtgcctcaggcaggtggagagccaga
aaggggcaggctcagcaatgtggaaatcctgggagccctgggaaagcagc
tcatctgtccctgctcctcactctcagctgagcaagatcctccaacaaca
gtgccttctaaaatgggaatcagtcctacctgggttgctcgggtcttgag
gcaaac

length: 506 bp

AMPLIMER	from	to
genomic DNA	1723	2228

Jump to exon 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | top

TNNT2 Exon 5

agcaggaggaggcagcggaagaggatgctgaagcagaggctgagaccgag
gagaccagggcagaag

length: 66 bp

EXON	from	to
genomic DNA	5842	5907
mRNA	118	183

Primers for Exon 5
TrpnT 5F gtggcgggaggtagccgacagt (22 bp)

TrpnT 5R tgggcaatcaatggttgaatctta (24 bp)

**Primers for Exon 5**
TrpnT 5F	gtggcgggaggtagccgacagt	(22 bp)
TrpnT 5R	tgggcaatcaatggttgaatctta	(24 bp)

(Annealing temp.: 65° C.)

Amplimer for Exon 5

gtggcgggaggtagccgacagtcaaaaccaagaatggcagccccccagca
cgttggttgcccccaaattcatctggacctagacgtcttgacccccatcc
ccactccccgctgcccattctctgctctgggttctgcctgatagcatgag
tggggcctgcttcttcactctgtctgcttcacttctccagagcaggagga
ggcagcggaagaggatgctgaagcagaggctgagaccgaggagaccaggg
cagaaggtaagtctctggttgagggtgcaggggcctcagggggtggcgag
agcccctggagccattcctgaggcagtaagaaaccccccagaacaggctt
tcccatgtgcacctgactctcctattgactaagattcaaccattgattgc
cca

length: 403 bp

AMPLIMER	from	to
genomic DNA	5652	6054

Jump to exon 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | top

TNNT2 Exon 6

aagatgaagaagaagaggaagcaaaggaggctgaag

length: 36 bp

EXON	from	to
genomic DNA	6263	6298
mRNA	184	219

Primers for Exon 6
TrpnT 6F ttgacccagcgcttctcttgtgtc (24 bp)

TrpnT 6R actgggtgccaccaatgcaacttc (24 bp)

**Primers for Exon 6**
TrpnT 6F	ttgacccagcgcttctcttgtgtc	(24 bp)
TrpnT 6R	actgggtgccaccaatgcaacttc	(24 bp)

(Annealing temp.: 65° C.)

Amplimer for Exon 6

ttgacccagcgcttctcttgtgtcaggcagtcttgggtccaggggaatgt
gtgtgtgagagacagctctgtaaccccaaaaaccaacagagtgcatcttg
gctagggcttatctgtggggaaggctcttgctggtggcggagggggggac
ttgagctcagaacaaggaccttgattgatttctcgtgtttcatttagaag
atgaagaagaagaggaagcaaaggaggctgaaggtaaggatatcaggaga
gaggtacaagggaggcaaaggaacccctcacagcagctttggaggagaat
gcccacagtgctctttccagggaagtggatgtgcactgagagggtttggg
agaggagaggacagaacttggtggcacttccaaagttttagatgttgagc
tttcaactttgccccttctcccagagaagttgcattggtggcacccagt

length: 449 bp

AMPLIMER	from	to
genomic DNA	6066	6514

Jump to exon 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | top

TNNT2 Exon 7

atggcccaatggaggagtccaaaccaaagcccag

length: 34 bp

EXON	from	to
genomic DNA	7198	7231
mRNA	220	253

Primers for Exon 7
TrpnT 7F ccagtgccgggagggactcac (21 bp)

TrpnT 7R cagcccgtgtccactgcaccatac (24 bp)

**Primers for Exon 7**
TrpnT 7F	ccagtgccgggagggactcac	(21 bp)
TrpnT 7R	cagcccgtgtccactgcaccatac	(24 bp)

(Annealing temp.: 65° C.)

Amplimer for Exon 7

ccagtgccgggagggactcactgtgcagatggggaaatggaaatccacag
ggatctagctcatcgagggcggagttgggaccacttctggggccccctga
cccatggctgtgtgcttctatctcctccagatggcccaatggaggagtcc
aaaccaaagcccaggtgagtggggaggcacctgggtagagccgggagcag
aggctcagggagagaggatgatgatggaacggggtgcagtatggtgcagt
ggacacgggctg

length: 262 bp

AMPLIMER	from	to
genomic DNA	7068	7329

Jump to exon 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | top

TNNT2 Exon 8

gtcgttcatgcccaacttggtgcctcccaagatccccgatggagagagag
tggactttgat

length: 61 bp

EXON	from	to
genomic DNA	8399	8459
mRNA	254	314

Primers for Exon 8
TrpnT 8F ggatcaggccctgcctgtcctgaca (25 bp)

TrpnT 8R tcctcctcctctttcttcctgttct (25 bp)

**Primers for Exon 8**
TrpnT 8F	ggatcaggccctgcctgtcctgaca	(25 bp)
TrpnT 8R	tcctcctcctctttcttcctgttct	(25 bp)

(Annealing temp.: 62° C.)

Amplimer for Exon 8

ggatcagggccctgcctgtcctggacaccctcagtccctgggtccagaat
ggggctgatgctgactattcctctctccaacaggtcgttcatgcccaact
tggtgcctcccaagatccccgatggagagagagtggactttgatgtaagc
ggtggctgtgggttgagtaggcctgggctggggatagtcccaagggccct
cagtgacagagattttgtgaccttgggctggggtagagcataggtgggat
ggggtgcagcaccagccaggctgggtgcccatccatgagcgtggtcctgg
gttcaggccacagttaccccttccctggccaggcgccaggccctgccaga
ggtcttttgcactgcgttggggggtgtctagcccacccatctctcctctg
gactctttggagtggcagcctctgagccgcagcggtccacccacaggaca
tccaccggaagcgcatggagaaggacctgaatgagttgcaggcgctgatc
gaggctcactttgagaacaggaagaaagaggaggagga

length: 538 bp

AMPLIMER	from	to
genomic DNA	8316	8853

Jump to exon 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | top

TNNT2 Exon 9

gacatccaccggaagcgcatggagaaggacctgaatgagttgcaggcgct
gatcgaggctcactttgagaacaggaagaaagaggaggaggagctcgttt
ctctcaaagacaggatc

length: 117 bp

EXON	from	to
genomic DNA	8762	8878
mRNA	315	431

Primers for Exon 9
TrpnT 9F gccaggccctgccagaggtctt (22 bp)

TrpnT 9R ccctgggggaggcctgaaacag (22 bp)

**Primers for Exon 9**
TrpnT 9F	gccaggccctgccagaggtctt	(22 bp)
TrpnT 9R	ccctgggggaggcctgaaacag	(22 bp)

(Annealing temp.: 70° C.)

Amplimer for Exon 9

GCCAGGCCCTGCCAGAGGTCTTttgcactgcgttggggggtgtctagccc
acccatctctcctctggactctttggagtggcagcctctgagccgcagcg
gtccacccacaggacatccaccggaagcgcatggagaaggacctgaatga
gttgcaggcgctgatcgaggctcactttgagaacaggaagaaagaggagg
aggagctcgtttctctcaaagacaggatcgtaggtgtccagtctgcccac
ctaacattctctgatggcccagtctgtcctccatcccttttgtgaggccc
cacctcctccagacagccttcctcggcgcctcaaacccagctaggtgtga
ggtctccttcagaggccctccagcattctgcacagctcctgcagccctga
ctggtttctcgggtccctgccttccctccccatcctactggatgctcctt
gactgccagagctgagtctgatCTGTTTCAGGCCTCCCCCAGGG

length: 494 bp

AMPLIMER	from	to
genomic DNA	8650	9143

Jump to exon 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | top

TNNT2 Exon 10

gagagacgtcgggcagagcgggccgagcagcagcgcatccggaatgagcg
ggagaaggagcggcagaaccgcctggct

length: 78 bp

EXON	from	to
genomic DNA	9694	9771
mRNA	432	509

Primers for Exon 10
TrpnT 10F acgtccgtggagctggttgaaagt (24 bp)

TrpnT 10R cccggccaatattgtctcttgact (24 bp)

**Primers for Exon 10**
TrpnT 10F	acgtccgtggagctggttgaaagt	(24 bp)
TrpnT 10R	cccggccaatattgtctcttgact	(24 bp)

(Annealing temp.: 62° C.)

Amplimer for Exon 10

ACGTCCGTGGAGCTGGTTGAAAGTGGAGGCCCTTGGAGGCCGGGCACCAT
TGCTTCAAGACTCTGGAAGGAACCCTCCCCCAGGAATTCCCTTGCCACCC
CATGCAGGTTTCTGTACCTGCGATGTCACCTTCTCCCTATGCACACCTGG
GTGGGCTTTGCCTCTTCTCCTTAGGAGAGACGTCGGGCAGAGCGGGCCGA
GCAGCAGCGCATCCGGAATGAGCGGGAGAAGGAGCGGCAGAACCGCCTGG
CTGTGAGTGACCCCTAGCCCAGGCCCATGGAGGCCCCCCATGCTCCCAGC
TCCTGCTGGGCCCCCAGGCCCCTCTCTATTCATCAACATGAATGAAATCA
GTCAAGAGACAATATTGGCCGGG

length: 373 bp

AMPLIMER	from	to
genomic DNA	9520	9892

Jump to exon 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | top

TNNT2 Exon 11

gaagagagggctcgacgagaggaggaggagaacaggaggaaggctgagga
tgaggcccggaagaagaaggctttgtccaacatgatgcattttgggggtt
acatccagaag

length: 111 bp

EXON	from	to
genomic DNA	10663	10773
mRNA	510	620

Primers for Exon 11
TrpnT 11 F tgggagctaccctctcagaa (20 bp)

TrpnT 11 R cacagcagctgggaatctct (20 bp)

**Primers for Exon 11**
TrpnT 11 F	tgggagctaccctctcagaa	(20 bp)
TrpnT 11 R	cacagcagctgggaatctct	(20 bp)

(Annealing temp.: 60° C.)

Amplimer for Exon 11

TGGGAGCTACCCTCTCAGAAAGCTCCTTGCTGAGCGGAGAGAAAGCTGAA
CTCACCCATAAAGACCACAAGCTTCAGCCCAGAATCAGGGTTTCCAATCC
TTTCCCCTAATTTGCTTTCTTCCTCCCTGCTGTAAATCAGGAAGAGAGGG
CTCGACGAGAGGAGGAGGAGAACAGGAGGAAGGCTGAGGATGAGGCCCGG
AAGAAGAAGGCTTTGTCCAACATGATGCATTTTGGGGGTTACATCCAGAA
GGTAGGTAGGGAGCAGCAGGGGTTGCCAGGAGATCCTAGTATAGCCCTGA
GGAATGAGGTGTCCACTGCAGCAGGTAGACTTTAGGTCAGGTCCCAGGAA
GAGATTCCCAGCTGCTGTG

length: 369 bp

AMPLIMER	from	to
genomic DNA	10523	10891

Jump to exon 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | top

TNNT2 Exon 12

caggcccag

length: 9 bp

EXON	from	to
genomic DNA	11675	11683
mRNA	621	629

Primers for Exon 12
TrpnT 12 F gtaaacccggctgactacag (20 bp)

TrpnT 12 R agccagcccaatctcttcac (20 bp)

**Primers for Exon 12**
TrpnT 12 F	gtaaacccggctgactacag	(20 bp)
TrpnT 12 R	agccagcccaatctcttcac	(20 bp)

(Annealing temp.: 62° C.)

Amplimer for Exon 12

GTAAACCCGGCTGACTACAGCGAGTATCTGAATCTTCTCTTCCATGTCTC
TCCTTGCCTTTGTCTTCCTGCCTTCTCTCTGTGTCTCTGTTGCCGTCTTG
GTCTTTTTCTATGGGCCTCTCTCTCATGGTTTCCCGGTTTAGCAGGCCCA
GGTGGGTACCAAAAACCTCAAGCCTTCTTCCTCCCCATAGTTTTGGAGAG
CTCCCTGGGAGGGGAAGGCGCCTTGCTGGAGGGGAGGAGTGAAGAGATTG
GGCTGGCT

length: 258 bp

AMPLIMER	from	to
genomic DNA	11533	11790

Jump to exon 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | top

TNNT2 Exon 13

acagagcggaaaagtgggaagaggcagactgagcgggaaaagaagaagaa
gattctggctgagaggaggaaggtgctggccattgaccacctgaatgaag
atcagctgag

length: 110 bp

EXON	from	to
genomic DNA	12047	12156
mRNA	630	739

Primers for Exon 13
TrpnT 13 F cagggggtttggggagggttag (22 bp)

TrpnT 13 R gtggggcacctgctcagttctct (23 bp)

**Primers for Exon 13**
TrpnT 13 F	cagggggtttggggagggttag	(22 bp)
TrpnT 13 R	gtggggcacctgctcagttctct	(23 bp)

(Annealing temp.: 60° C.)

Amplimer for Exon 13

CAGGGGGTTTGGGGAGGGTTAGGGGACAGGGAGGGGGCAATCTGGCCAGT
TTACTCTGCTTCCCACACCCTCCAGACAGAGCGGAAAAGTGGGAAGAGGC
AGACTGAGCGGGAAAAGAAGAAGAAGATTCTGGCTGAGAGGAGGAAGGTG
CTGGCCATTGACCACCTGAATGAAGATCAGCTGAGGTGGGGGCTGGGAGG
AATGGTCCTGGGGCACTGCCCGCATCTGCTCTGGGAGGGGTCGGGCCAAG
GGCACTTCTTGCTGTGAGCCACCAGAGGGCAGGACTGCCTCACATATTTT
CCCATGGCAAGTTTGGTGGGACCAGAGCTGGGCCCCAGCAGGTCGGGCCA
ACTCCTTTCTGGGCTTTGCTGATGGGGAAAGAGAACTGAGCAGGTGCCCC
AC

length: 402 bp

AMPLIMER	from	to
genomic DNA	11972	12373

Jump to exon 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | top

TNNT2 Exon 14

ggagaaggccaaggagctgtggcagagcatctataacttggaggcagaga
agttcgacctgcaggagaagttcaagcagcagaaatatgag

length: 91 bp

EXON	from	to
genomic DNA	12700	12790
mRNA	740	830

Primers for Exon 14
TrpnT 14F ggagggccctttcttactggac (22 bp)

TrpnT 14R ccggacccagtgaaccaggaggag (24 bp)

**Primers for Exon 14**
TrpnT 14F	ggagggccctttcttactggac	(22 bp)
TrpnT 14R	ccggacccagtgaaccaggaggag	(24 bp)

(Annealing temp.: 68° C.)

Amplimer for Exon 14

GGAGGGCCCTTTCTTACTGGACCTCCCCATTCTCACCCTTGCCCGTGCAG
GGAGAAGGCCAAGGAGCTGTGGCAGAGCATCTATAACTTGGAGGCAGAGA
AGTTCGACCTGCAGGAGAAGTTCAAGCAGCAGAAATATGAGGTGGGCCGC
CATGCTGTCCCCGCCCAGCATCTCCATCTCACACTCCTCCTGGTTCACTG
GGTCCGG

length: 207 bp

AMPLIMER	from	to
genomic DNA	12650	12856

Jump to exon 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | top

TNNT2 Exon 15

atcaatgttctccgaaacaggatcaacgataaccagaaagt

length: 41 bp

EXON	from	to
genomic DNA	14406	14446
mRNA	831	871

Primers for Exon 15
TrpnT 15F gcccctcctgacccttaactatcc (24 bp)

TrpnT 15R cggaggagccagagaaggaaacct (24 bp)

**Primers for Exon 15**
TrpnT 15F	gcccctcctgacccttaactatcc	(24 bp)
TrpnT 15R	cggaggagccagagaaggaaacct	(24 bp)

(Annealing temp.: 62° C.)

Amplimer for Exon 15

GCCCCTCCTGACCCTTAACTATCCtaacccctcctactcttccatgctcc
tccttctcctcctgcactgctgcactcagcccccttctccccatccccag
gccacctgggacctgagccagtcagctccagcgttgctctttgtccttcc
cacttttcttgcagatcaatgttctccgaaacaggatcaacgataaccag
aaagtgtaagtgtctgaggtcattctcgcctagccctccccctgccccct
ccttccctaccttccagctatcccattcctccttggagggccccaggctc
ccagcccttcctcctctgcttcagcccacAGGTTTCCTTCTCTGGCTCCT
CCG

length: 353 bp

AMPLIMER	from	to
genomic DNA	14242	14594

Jump to exon 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | top

TNNT2 Exon 16

ctccaagacccgcgggaaggctaaagtcaccgggcgctggaaatag

length: 46 bp

EXON	from	to
genomic DNA	14814	14859
mRNA	872	917

Primers for Exon 16
TrpnT 16F gggggtgaaatgtggggcggagaa (24 bp)

TrpnT 16R gtgtgggggcaggcaggagtggtg (24 bp)

**Primers for Exon 16**
TrpnT 16F	gggggtgaaatgtggggcggagaa	(24 bp)
TrpnT 16R	gtgtgggggcaggcaggagtggtg	(24 bp)

(Annealing temp.: 62° C.)

Amplimer for Exon 16

GGGGGTGAAATGTGGGGCGGAGAAgcttccctccagcctcaggctgctgt
cctattctgcccctggaggcagctccagcctgctcctctcccctttggca
ccccagtcctaccccagccgcatggtgacctactaccctgcctgtgtctc
catgtcactgcgtcctgcttcccctgcagctccaagacccgcgggaaggc
taaagtcaccgggcgctggaaatagagcctggcctccttcaccaaagatc
tgctcctcgctcgcacctgcctccggcctgcactcccccagttcccgggc
cctcctgggcaccccaggcagctcctgtttggaaatggggagctggccta
ggtgggagcCACCACTCCTGCCTGCCCCCACAC

length: 383 bp

AMPLIMER	from	to
genomic DNA	14635	15017

Jump to exon 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | top

citations & disclaimer | contact us

Last modified: April 24, 2006