TNNI3 exons, primers, and amplimers

Exons, Primers, and Amplimers of cardiac Troponin I

Jump to exon 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | top

TNNI3 Exon 1

gtctcagcATGgcggatggg

length: 20 bp

EXON	from	to
genomic DNA	1242	1260
mRNA	82	101

Primers for Exon 1
TrpnI 1F tcacgcgcccccgttatctgg (21 bp)

TrpnI 1R gacccctggagtcccctatgaaca (24 bp)

**Primers for Exon 1**
TrpnI 1F	tcacgcgcccccgttatctgg	(21 bp)
TrpnI 1R	gacccctggagtcccctatgaaca	(24 bp)

(Annealing temp.: 65° C.)

Amplimer for Exon 1

tcacgcgcccccgttatctggcatagtgggcagggggaggaggaggtgac
agtatatttagtctgtgtcctcgccctttatctcagtgtcctcggggagt
ctcaagcagcccggaggagactgacggtccctgggaccctgaaggtcacc
cgggcggccccctcactgaccctccaaacgcccctgtcctcgccctgcct
cctgccattcccggcctgagtctcagcatggcggatgggtgagtgatgcc
ccaaggcagtggggagttgggggcgacctcccgggttcccaagaggggtc
gcagctgagaggctggacccaaggcctgcgaggatggcgtagggactctt
ccctgaagagaggaagtgggtttgcgagtcagactcctggaacccaagga
aggggaagctcggtcccccgacctcttgttcataggggactccaggggtc

length: 450 bp

AMPLIMER	from	to
genomic DNA	1023	1472

Jump to exon 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | top

TNNI3 Exon 2

agcagcgatgcg

length: 12 bp

EXON	from	to
genomic DNA	1532	1544
mRNA	102	113

Primers for Exon 2
TrpnI 2F agcatggcggatgggtgagtgat (23 bp)

TrpnI 2R caagaccccacccagcccttacc (23 bp)

**Primers for Exon 2**
TrpnI 2F	agcatggcggatgggtgagtgat	(23 bp)
TrpnI 2R	caagaccccacccagcccttacc	(23 bp)

(Annealing temp.: ° C.)

Amplimer for Exon 2

AGCATGGCGGATGGGTGAGTGATGCCCCAAGGCAGTGGGGAGTTGGGGGC
GACCTCCCGGGTTCCCAAGAGGGGTCGCAGCTGAGAGGCTGGACCCAAGG
CCTGCGAGGATGGCGTAGGGACTCTTCCCTGAAGAGAGGAAGTGGGTTTG
CGAGTCAGACTCCTGGAACCCAAGGAAGGGGAAGCTCGGTCCCCCGACCT
CTTGTTCATAGGGGACTCCAGGGGTCCCTTAGGAGACAGGACACAGCCCA
CCACTAACCCCCCTCCTTGGTTTCTCTCCTTCCAGGAGCAGCGATGCGGT
GAGAGCAGCGGGCTAAGGCGTGGCTGGGACCCCCAGGTCCAGGGTGGGCC
CTGCAGTGAGGGGTCTGGGTCGGGAGGCTGCAGCCTAGCAGAGGGTGCGG
TACGGTAAGGGCTGGGTGGGGTCTTG

length: 426 bp

AMPLIMER	from	to
genomic DNA	1247	1672

Jump to exon 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | top

TNNI3 Exon 3

gctagggaacctcgccctgcaccagccccaatcagacgccgctcctccaa
ctaccgcgcttatgccacggagccgcacgccaag

length: 85 bp

EXON	from	to
genomic DNA	1706	1789
mRNA	114	197

Primers for Exon 3
TrpnI 3F tagcagagggtgcggtacggtaag (24 bp)

TrpnI 3R ccgagggcaacggagttcc (19 bp)

**Primers for Exon 3**
TrpnI 3F	tagcagagggtgcggtacggtaag	(24 bp)
TrpnI 3R	ccgagggcaacggagttcc	(19 bp)

(Annealing temp.: 65° C.)

Amplimer for Exon 3

tagcagagggtgcggtacggtaagggctgggtggggtcttggtggtcatg
gggtccccactcctcctaacccaggctagggaacctcgccctgcaccagc
cccaatcagacgccgctcctccaactaccgcgcttatgccacggagccgc
acgccaaggtgggacggggcttcctgggggcagagtacaggcgccggagg
gatccaagaccctgggagtggggggaggagccagggctgcgaagggggcg
gggactacgcggaggggcttcaggggcggagttttgcagagggtcatgct
cggattggtgacagcagcctgcgggcggaactccgttgccctcgg

length: 345 bp

AMPLIMER	from	to
genomic DNA	1632	1976

Jump to exon 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | top

TNNI3 Exon 4

aaaaaatctaagatctccgcctcgagaaaattgcagctgaag

length: 42 bp

EXON	from	to
genomic DNA	2195	2236
mRNA	198	239

Primers for Exon 4
TrpnI 4F gggcggggcctcccgtagac (20 bp)

TrpnI 4R taccccgaaagccccacccattc (23 bp)

**Primers for Exon 4**
TrpnI 4F	gggcggggcctcccgtagac	(20 bp)
TrpnI 4R	taccccgaaagccccacccattc	(23 bp)

(Annealing temp.: 65° C.)

Amplimer for Exon 4

gggcggggcctcccgtagactcagggctcaagttggacgtgggcccaaat
ctggaccggctgggtttgctgggggtgtcttgaggtcccctccaccgtcg
tctccgaatccccctccatgatccttccttgctccatctcaccctggcag
aaaaaatctaagatctccgcctcgagaaaattgcagctgaaggtgaggac
gggcgggactgggaaagagcaggcaggtgctcagggggcggacgttgaga
atgggtggggctttcggggta

length: 271 bp

AMPLIMER	from	to
genomic DNA	2045	2315

Jump to exon 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | top

TNNI3 Exon 5

actctgctgctgcagattgcaaagcaagagctggagcgagaggcggagga
gcggcgcggagagaaggggcgcgctctgagcacccgctgccagccgctgg
agttggccgggctgggcttcgcggagctgcag

length: 132 bp

EXON	from	to
genomic DNA	2507	2638
mRNA	240	371

Primers for Exon 5
TrpnI 5F acggggccttggaacagtggaga (23 bp)

TrpnI 5R cccgagcagaagaggggatagag (23 bp)

**Primers for Exon 5**
TrpnI 5F	acggggccttggaacagtggaga	(23 bp)
TrpnI 5R	cccgagcagaagaggggatagag	(23 bp)

(Annealing temp.: 65° C.)

Amplimer for Exon 5

acggggccttggaacagtggagaccaaactggagggtttagaagggcaga
ggcggttccccacgcctggtctttatcctgaagccccgggtggcgtgcgc
ttccctcccacccctctgcagactctgctgctgcagattgcaaagcaaga
gctggagcgagaggcggaggagcggcgcggagagaaggggcgcgctctga
gcacccgctgccagccgctggagttggccgggctgggcttcgcggagctg
caggtaccggctcccaaggatgcgaggtttctagtcccggaattcagcag
tacagcctctatcccctcttctgctcggg

length: 329 bp

AMPLIMER	from	to
genomic DNA	2386	2714

Jump to exon 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | top

TNNI3 Exon 6

gacttgtgccgacagctccacgcccgtgtggacaaggtggatgaagagag
atacgacatagaggcaaaagtcaccaagaacatcacggag

length: 90 bp

EXON	from	to
genomic DNA	4009	4098
mRNA	372	461

Primers for Exon 6
TrpnI 6F acccaggagtccaggtctc (19 bp)

TrpnI 6R aagtcccagccatctcaccctacc (24 bp)

**Primers for Exon 6**
TrpnI 6F	acccaggagtccaggtctc	(19 bp)
TrpnI 6R	aagtcccagccatctcaccctacc	(24 bp)

(Annealing temp.: 65° C.)

Amplimer for Exon 6

acccaggagtccaggtctccctgtttttggttcccccaacaacacacacc
acgttcctcctccaggacttgtgccgacagctccacgcccgtgtggacaa
ggtggatgaagagagatacgacatagaggcaaaagtcaccaagaacatca
cggaggtgggacgcatgggcagctcgggtaccttcggggtagggtgagat
ggctgggactt

length: 211 bp

AMPLIMER	from	to
genomic DNA	3944	4154

Jump to exon 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | top

TNNI3 Exon 7

attgcagatctgactcagaagatctttgaccttcgaggcaagtttaagcg
gcccaccctgcggagagtgaggatctctgcagatgccatgatgcaggcgc
tgctgggggcccgggctaaggagtccctggacctgcgggcccacctcaag
caggtgaagaaggaggacaccgagaag

length: 177 bp

EXON	from	to
genomic DNA	4633	4809
mRNA	462	638

Primers for Exon 7
TrpnI 7F gcctaagccgggaagagactggta (24 bp)

TrpnI 7R gaggaccccttactagctgcttct (24 bp)

**Primers for Exon 7**
TrpnI 7F	gcctaagccgggaagagactggta	(24 bp)
TrpnI 7R	gaggaccccttactagctgcttct	(24 bp)

(Annealing temp.: 65° C.)

Amplimer for Exon 7

gcctaagccgggaagagactggtaaggcctcggtactggaagacgagata
aggagaataaaaaaggagtgtaggatggaggagttgggtgtgcgggaaat
ggaaggagaagtacccaccccctcgtgtgcccccagattgcagatctgac
tcagaagatctttgaccttcgaggcaagtttaagcggcccaccctgcgga
gagtgaggatctctgcagatgccatgatgcaggcgctgctgggggcccgg
gctaaggagtccctggacctgcgggcccacctcaagcaggtgaagaagga
ggacaccgagaaggtgagtgtgggctaaggccaggaaagaggatgctgag
gggaagggctgtgggtgccaacaaccctaggcctgagggcagatggtgct
tggagttggaggtagaagcagctagtaaggggtcctc

length: 437 bp

AMPLIMER	from	to
genomic DNA	4497	4933

Jump to exon 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | top

TNNI3 Exon 8

gaaaaccgggaggtgggagactggcgcaagaacatcgatgcactgagtgg
aatggagggccgcaagaaaaagtttgagagctgagccttcctgcctactg
cccctgccctgaggagggccctgaggaataaagcttctctctgagctgaa
a

length: 151 bp

EXON	from	to
genomic DNA	6442	6592
mRNA	639	787

Primers for Exon 8
TrpnI 8F tggggaaaattggcagggattatc (24 bp)

TrpnI 8R cctcctcagggcaggggcagtag (23 bp)

**Primers for Exon 8**
TrpnI 8F	tggggaaaattggcagggattatc	(24 bp)
TrpnI 8R	cctcctcagggcaggggcagtag	(23 bp)

(Annealing temp.: 65° C.)

Amplimer for Exon 8

tggggaaaattggcagggattatcttgaaaagacaggaagtgctccagaa
ctagatacttaggcatccagggtagagtggccccacaggctggagggaag
acagggattcttgagagactggagaccaagaagagaccctaacctctgac
tcatcgccatcctccaggaaaaccgggaggtgggagactggcgcaagaac
atcgatgcactgagtggaatggagggccgcaagaaaaagtttgagagctg
agccttcctgcctactgcccctgccctgaggagg

length: 284 bp

AMPLIMER	from	to
genomic DNA	6275	6558

Jump to exon 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | top

citations & disclaimer | contact us

Last modified: April 24, 2006