B.2 b-gal Solution Assay : for quantitative analysis
C.1 CsCl Prep. of Plasmid DNA
C.2 Cupric Chloride Staining : for SDS-PAGE gels
C.3 Chromosomal DNA Prep : cultured cells/tissue samples
C.4 Confocal Methods : HMS Genetics Leica confocal
C.5 COS cell Transfection
C.6 Conditioned Medium : for retinal explant cultures
C.7 Cryosectioning
D.1 DNA Plasmid Prep. : Baron Lab long version
D.2 Quick DNA Plasmid Prep. : boiling 10 minute protocol
D.3 Lambda DNA Prep.
D.4 Quick DNA Plasmid Prep. : NaOH/SDS protocol
D.5 DNA Fragment Purif. : for agarose & acrylamide
D.6 Dissociated Cell Staining : immunohistochemistry
E.1 EMSA Using Oligos
E.2 Electroelution of Proteins : includes renaturing
F.1 Fill-in Labeling of DNA : directional end label
F.2 DNaseI Footprinting
F.3 FCC & Transformation : standard protocol
F.4 FCC & Transformation (B) : high efficiency
G.1 GuHCl Purification : for SDS-PAGE gels
G.2 Gel Recipes
H.1 His Tagged Proteins : for E. coli expression
H.2 Histochemical Staining : AP and Beta-galactosidase
L.1 Lambda gt22a Screening : for DNA Binding Proteins
M.1 Methylation Interference
M.2 Maxam & Gilbert Seq. : Quick Protocol for F.2
N.1 Nuclear Extract Prep.
O.1 Oligonucleotide Purif. : PAGE
P.1 Protein Gels
P.2 Ponceau Staining
P.3 PCR : representative reaction
P.4 PCR Genotype : tail prep. DNA
P.5 Parrafin Sectioning
R.1 Random Prime Labeling : for blots etc...
R.2 RNA Preparation : cultured cells & tissue
R.3 RNase Protection Assay
R.4 RT-PCR : semi-quantitative
S.1 Sequencing : use with protocol D.2
S.2 ssDNA Preparation : for sequencing
S.3 Southwestern Blot : for DNA binding proteins
S.4 Stable Cell Lines
S.5 Southern Blotting : includes NENSORB
S.6 Silinazation of Slides
T.1 Tmax Film Developing
T.2 Taq Polymerase Purif.
T.3 [³H] Thymidine Labeling
T.4 Tyramide Amplification : includes synthesis
V.1 Viral Production : includes titering
V.2 Viral Production (B) : Gary Nolan’s protocol
W.1 Western Blotting